Adress: H-1111 Szent Gellért tér 4. Budapest, Hungary, BME Building Ch, 1st floor, “Déli 1 Laboratory”

Phone: +361 463 1129

e-mail: info@nanobiotech.hu

1. Balogh-Weiser, Diána ✉ ; Molnár, Alexandra ; Tóth, Gergő D. ; Koplányi, Gábor ; Szemes, József ; Decsi, Balázs ; Katona, Gábor ; Salamah, Maryana ; Ender, Ferenc ; Kovács, Anita et al.
   Combined Nanofibrous Face Mask: Co-Formulation of Lipases and Antibiotic Agent by Electrospinning Technique
   PHARMACEUTICS 15 : 4 Paper: 1174 , 19 p. (2023)
2. Csorba, Anita ; Katona, Gábor ; Budai-Szűcs, Mária ; Balogh-Weiser, Diána ; Fadda, Anna Maria ; Caddeo, Carla ; Takács, Ágnes Ildikó ; Mátyus, Péter ; Balogh, György T. ✉ ; Nagy, Zoltán Zsolt ✉, Effect of liposomal formulation of ascorbic acid on corneal permeability, SCIENTIFIC REPORTS 13 : 1 Paper: 3448 , 12 p. (2023)
3. Imarah, Ali O. ; Silva, Fausto M. W. G. ; Bataa, Naran ; Decsi, Balázs ; Balogh-Weiser, Diána ; Poppe, László ✉
   Magnetically agitated continuous-flow tube reactors with aspartate ammonia-lyase immobilized on magnetic nanoparticles
   REACTION CHEMISTRY & ENGINEERING 8 (2023)
4. Koplányi, Gábor ; Bell, Evelin ; Molnár, Zsófia ; Katona, Gábor ; Neumann, Péter Lajos ; Ender, Ferenc ; Balogh, György Tibor; Žnidaršič-Plazl, Polona ; Poppe, László ; Balogh-Weiser, Diana ✉Novel Approach for the Isolation and Immobilization of a Recombinant Transaminase: Applying an Advanced Nanocomposite System
   CHEMBIOCHEM 24 : 7 Paper: e202200713 , 11 p. (2023)
5. Vincze, Anna ; Dékány, Gergely ; Bicsak, Richárd ; Formanek, András ; Moreau, Yves ; Koplányi, Gábor ; Takács, Gergely ; Katona, Gábor ; Balogh-Weiser, Diána ; Arany, Ádám et al.
   Natural Lipid Extracts as an Artificial Membrane for Drug Permeability Assay: In Vitro and In Silico Characterization
   PHARMACEUTICS 15 : 3 Paper: 899 , 19 p. (2023)
6. Balogh, György T. ✉ ; Decsi, Balázs ; Krammer, Réka ; Kenéz, Balázs ; Ender, Ferenc ; Hergert, Tamás ; Balogh-Weiser, Diána ✉Effect of Binding Linkers on the Efficiency and Metabolite Profile of Biomimetic Reactions Catalyzed by Immobilized Metalloporphyrin
   METABOLITES 12 : 12 p. 1269 , 13 p. (2022)
7. Balogh-Weiser, Diána ✉ ; Poppe, László ✉ ; Kenéz, Balázs ; Decsi, Balázs ; Koplányi, Gábor ; Katona, Gábor ; Gyarmati, Benjámin ; Ender, Ferenc ; Balogh, György T. ✉Novel biomimetic nanocomposite for investigation of drug metabolism, JOURNAL OF MOLECULAR LIQUIDS 368 : Part B Paper: 120781 , 10 p. (2022)
1. Csuka, Pál ; Molnár, Zsófia* ; Tóth, Veronika ; Imarah, Ali Obaid ; Balogh-Weiser, Diána ; Vértessy, Beáta G ; Poppe, Laszlo ✉, Immobilization of the Aspartate Ammonia‐lyase from Pseudomonas fluorescens R124 on Magnetic Nanoparticles − Characterization and Kinetics, CHEMBIOCHEM (2022)
2. Nagy, Flóra ; Sánta-Bell, Evelin ; Jipa, Monica ; Hornyánszky, Gábor ; Szilágyi, András ; László, Krisztina ; Katona, Gábor ; Paizs, Csaba ; Poppe, László ✉ ; Balogh-Weiser, Diána ✉, Cross-Linked Enzyme-Adhered Nanoparticles (CLEANs) for Continuous-Flow Bioproduction, CHEMSUSCHEM 15 : 2 Paper: e202102284 (2022)
3. Gal, Cristian Andrei ; Barabas, Laura Edit ; Bartha-Vari, Judith Hajnal ; Moisa, Madalina Elena ; Balogh-Weiser, Diana ; Bencze, Laszlo Csaba ; Poppe, László ; Paizs, Csaba ; Tosa, Monica Ioana ✉ Lipase on carbon nanotube – An active, selective, stable and easy to optimize nanobiocatalyst for kinetic resolutions, REACTION CHEMISTRY & ENGINEERING 6 : 12 pp. 2391-2399. , 9 p. (2021)
4. Imarah, Ali Obaid ; Csuka, Pál ; Bataa, Naran ; Decsi, Balázs ; Sánta-Bell, Evelin ; Molnár, Zsófia ; Balogh-Weiser, Diána ; Poppe, László ✉, Magnetically Agitated Nanoparticle-Based Batch Reactors for Biocatalysis with Immobilized Aspartate Ammonia-Lyase, CATALYSTS 11 : 4 Paper: 483 , 13 p. (2021)
5. Juhász, Kinga ; Lévay, Krisztina ; Hegedűs, László ; Balogh-Weiser, Diána ; Pirault-Roy, Laurence ; Hell, Zoltán ✉, Application of supported lanthanum catalysts in the hydrogenation of nitriles, REACTION KINETICS MECHANISMS AND CATALYSIS 133 : 2 pp. 687-698. , 12 p. (2021)
6. Koplányi, Gábor ; Sánta-Bell, Evelin ; Molnár, Zsófia ; Tóth, Gergő Dániel ; Józó, Muriel ; Szilágyi, András ; Ender, Ferenc ; Pukánszky, Béla ; Vértessy, Beáta G. ; Poppe, László et al., Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning, CATALYSTS 11 : 10 Paper: 1149 , 14 p. (2021)
7. Tóth, Gergő Dániel ; Kazsoki, Adrienn ; Gyarmati, Benjámin ; Szilágyi, András ; Vasvári, Gábor ; Katona, Gábor ; Szente, Lajos ; Zelkó, Romána ; Poppe, László ; Balogh-Weiser, Diána ✉ et al.Nanofibrous Formulation of Cyclodextrin Stabilized Lipases for Efficient Pancreatin Replacement Therapies, PHARMACEUTICS 13 : 7 Paper: 972 , 18 p. (2021)
8. Diána, Balogh-Weiser ✉ ; Balázs, Decsi ; Réka, Krammer ; Gergő, Dargó ; Ferenc, Ender ; János, Mizsei ; Róbert, Berkecz ; Benjámin, Gyarmati ; András, Szilágyi ; Róbert, Tőtős et al., Magnetic Nanoparticles with Dual Surface Functions—Efficient Carriers for Metalloporphyrin-Catalyzed Drug Metabolite Synthesis in Batch and Continuous-Flow Reactors, NANOMATERIALS 10 Paper: 2329 (2020)
9. Hegyesi, Nora ✉ ; Hodosi, Elek ; Polyak, Peter ; Faludi, Gabor ; Balogh-Weiser, Diana ; Pukanszky, Bela, Controlled degradation of poly-epsilon-caprolactone for resorbable scaffolds, COLLOIDS AND SURFACES B: BIOINTERFACES 186 Paper: 110678 , 7 p. (2020)
10. Kazsoki, Adrienn ; Farkas, Attila ; Balogh-Weiser, Diána ; Mancuso, Elena ; Sharma, Preetam K. ; Lamprou, Dimitrios A. ; Zelkó, Romána ✉, Novel combination of non-invasive morphological and solid-state characterisation of drug-loaded core-shell electrospun fibres, INTERNATIONAL JOURNAL OF PHARMACEUTICS 587 Paper: 119706 , 8 p. (2020)
11. Nagy, Flóra ; Gyujto, Imre ; Tasnádi, Gábor ; Barna, Bence ; Balogh-Weiser, Diána ; Faber, Kurt ; Poppe, László ✉ ; Hall, Mélanie ✉, Design and application of a bi-functional redox biocatalyst through covalent co-immobilization of ene-reductase and glucose dehydrogenase, JOURNAL OF BIOTECHNOLOGY 323 pp. 246-253. , 8 p. (2020)
12. Nagy-Győr, Lászó ; Farkas, Emese ; Lăcătuș, Mihai ; Tóth, Gergő ; Incze, Dániel ; Hornyánszky, Gábor ; Bódai, Viktória ; Paizs, Csaba ; Poppe, László ; Balogh-Weiser, Diána ✉, Conservation of the biocatalytic activity of whole yeast cells by supported sol-gel entrapment for efficient acyloin condensation, PERIODICA POLYTECHNICA-CHEMICAL ENGINEERING 64 : 2 pp. 153-161. , 9 p. (2020)
13. Decsi, Balázs ; Krammer, Réka ; Hegedűs, Kristóf ; Ender, Ferenc ; Gyarmati, Benjámin ; Szilágyi, András ; Tőtős, Róbert ; Katona, Gabriel ; Paizs, Csaba ; Balogh, György T. et al., Liver-on-a-Chip‒Magnetic nanoparticle bound synthetic metalloporphyrin-catalyzed biomimetic oxidation of a drug in a magnechip reactor, MICROMACHINES 10 : 10 Paper: 668 , 13 p. (2019)
14. Krisch, Enikő ; Balogh-Weiser, Diána ; Klimkó, Júlia ; Gyarmati, Benjámin ; László, Krisztina ; Poppe, László ✉ ; Szilágyi, András ✉ Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of enzymes, EXPRESS POLYMER LETTERS 13 : 6 pp. 512-523. , 12 p. (2019)
15. László, Nagy-Győr ; Mihai, Andrei Lacatus ; Diana, Balogh-Weiser ; Pál, Csuka ; Viktória, Bódai ; Balázs, Erdélyi ; Zsófia, Molnár ; Gabor, Hornyanszky ; Csaba, Paizs ✉ ; László, Poppe ✉, How to Turn Yeast Cells into a Sustainable and Switchable Biocatalyst? On-Demand Catalysis of Ketone Bioreduction or Acyloin Condensation, ACS SUSTAINABLE CHEMISTRY & ENGINEERING 7 : 24 pp. 19375-19383. , 9 p. (2019)
16. Sánta-Bell, Evelin ; Molnár, Zsófia ; Varga, Andrea ; Nagy, Flóra ; Hornyánszky, Gábor ; Paizs, Csaba ; Balogh-Weiser, Diána ✉ ; Poppe, László ✉, “Fishing and hunting”– Selective immobilization of a recombinant phenylalanine ammonia-lyase from fermentation media, MOLECULES 24 : 22 Paper: 4146 (2019)
17. Szűcs, Rózsa ; Balogh-Weiser, Diána* ; Sánta-Bell, Evelin ; Tóth-Szeles, Eszter ; Varga, Tamás ; Kónya, Zoltán ; Poppe, László ; Lagzi, István ✉ Green synthesis and in-situ immobilization of gold nanoparticles and their application for the reduction of p-nitrophenol in continuous-flow mode, RSC ADVANCES 9 : 16 pp. 9193-9197. , 5 p. (2019)
18. Balogh-Weiser, Diána, Enzimrögzítési módszerek nanostrukturált rendszerekben, MAGYAR KÉMIAI FOLYÓIRAT – KÉMIAI KÖZLEMÉNYEK (1997-) 124 : 1-2 pp. 49-55. , 7 p. (2018)
19. Kazsoki, Adrienn ; Szabó, Péter ; Domján, Attila ; Balázs, Attila ; Bozó, Tamás ; Kellermayer, Miklós ; Farkas, Attila ; Balogh-Weiser, Diána ; Pinke, Balázs ; Darcsi, András et al., Microstructural distinction of electrospun nanofibrous drug delivery systems formulated with different excipients, MOLECULAR PHARMACEUTICS 15 : 9 pp. 4214-4225. , 12 p. (2018)
20. Nagy, Flóra ; Tasnádi, Gábor ; Balogh Weiser, Diána ; Bell, Evelin ; Hall, Melanie ; Faber, Kurt ✉ ; Poppe, László ✉, Smart nanoparticles for selective immobilization of acid phosphatases, CHEMCATCHEM 10 : 16 pp. 3490-3499. , 10 p. (2018)
21. Nagy-Győr, László ; Abaházi, Emese ; Bódai, Viktória ; Sátorhelyi, Péter ; Erdélyi, Balázs ; Balogh-Weiser, Diána ; Paizs, Csaba ; Hornyánszky, Gábor ✉ ; Poppe, László ✉, Co-immobilized whole-cells with ω-transaminase and ketoreductase activity for continuous-flow cascade reaction, CHEMBIOCHEM 19 : 17 pp. 1845-1848. , 4 p. (2018)
22. Nóra, Adányi ; Ádám, György Nagy ; Bettina, Takács ; István, Szendrő ; George, Szakacs ; Rózsa, Szűcs ; Eszter, Tóth-Szeles ; István, Lagzi ; Diána, Weiser ; Viktória, Bódai et al., Sensitivity enhancement for mycotoxin determination by optical waveguide lightmode spectroscopy using gold nanoparticles of different size and origin, FOOD CHEMISTRY 267 pp. 10-14. , 5 p. (2018)
23. Tamás, Fődi ; Gergő, Ignácz ; Balázs, Decsi ; Zoltán, Béni ; György, István Túrós ; József, Kupai ; Diána, Balogh-Weiser ; István, Greiner ; Péter, Huszthy ; György, T. Balogh ✉, Biomimetic synthesis of drug metabolites in batch and continuous‐flow reactors,CHEMISTRY-A EUROPEAN JOURNAL 24 : 37 pp. 9385-9392. , 8 p. (2018)
24. Ender, Ferenc ; Weiser, Diána ; Vitéz, András ; Sallai, Gábor ; Németh, Márton ; Poppe, László ✉, In-situ measurement of magnetic nanoparticle quantity in a microfluidic device, MICROSYSTEM TECHNOLOGIES 23 : 9 pp. 3979-3990. , 12 p. (2017)
25. Weiser, Diána ; Nagy, Flóra ; Bánóczi, Gergely ; Oláh, Márk ; Farkas, Attila ; Szilágyi, András ; László, Krisztina ; Gellért, Ákos ; Marosi, György ; Kemény, Sándor et al., Immobilization engineering – How to design advanced sol-gel systems for biocatalysis?, GREEN CHEMISTRY 19 : 16 pp. 3927-3937. , 11 p. (2017)
26. Ender, Ferenc ✉ ; Diána, Weiser ; Nagy, Botond ; Bencze, László Csaba ; Paizs, Csaba ; Pálovics, Péter ; Poppe, László ✉, Microfluidic multiple cell chip reactor filled with enzyme-coated magnetic nanoparticles: An efficient and flexible novel tool for enzyme catalyzed biotransformation, JOURNAL OF FLOW CHEMISTRY 6 : 1 pp. 43-52. , 10 p. (2016)
27. Falus, Péter ; Cerioli, Lorenzo ; Bajnóczi, Gabriella ; Boros, Zoltán ; Weiser, Diána ; Nagy, József ; Tessaro, Davide ; Servi, Stefano ; Poppe, László ✉, A Continuous-Flow Cascade Reactor System for Subtilisin A-Catalyzed Dynamic Kinetic Resolution of N-tert-Butyloxycarbonylphenylalanine Ethyl Thioester with Benzylamine, ADVANCED SYNTHESIS & CATALYSIS 358 : 10 pp. 1608-1617. , 10 p. (2016)
28. Gihaz, Shalev ; Weiser, Diána ; Dror, Adi ; Sátorhelyi, Péter ; Jerabek-Willemsen, Moran ; Poppe, László ✉ ; Fishman, Ayelet ✉, Creating an efficient methanol-stable biocatalyst by protein and immobilization engineering–steps towards efficient biosynthesis of biodiesel, CHEMSUSCHEM 9 : 22 pp. 3161-3170. , 10 p. (2016)
29. Sóti, Péter Lajos ✉ ; Weiser, Diána ; Vigh, Tamás ; Nagy, Zsombor Kristóf ; Poppe, László ; Marosi, György, Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases, BIOPROCESS AND BIOSYSTEMS ENGINEERING 39 : 3 pp. 449-459. , 11 p. (2016)
30. Weiser, Diána ; Sóti, Péter L ; Bánóczi, Gergely ; Bódai, Viktória ; Kiss, Bálint ; Gellért, Ákos ; Nagy, Zsombor K ; Koczka, Béla ; Szilágyi, András ; Marosi, György et al., Bioimprinted lipases in PVA nanofibers as efficient immobilized biocatalysts, TETRAHEDRON 72 : 46 pp. 7335-7342. , 8 p. (2016)
31. Bartha-Vári, J H ; Toşa, M I ; Irimie, F -D ; Weiser, D ; Boros, Z ; Vértessy, B G ; Paizs, C ✉ ; Poppe, L ✉, Immobilization of Phenylalanine Ammonia‐Lyase on Single‐Walled Carbon Nanotubes for Stereoselective Biotransformations in Batch and Continuous‐Flow Modes, CHEMCATCHEM 7 : 7 pp. 1122-1128. , 7 p. (2015)
32. Weiser, D ; Bencze, L C ; Bánóczi, G ; Ender, F ; Kiss, R ; Kókai, E ; Szilágyi, A ; Vértessy, B G ; Farkas, Ö ; Paizs, C et al., Phenylalanine ammonia-lyase catalyzed deamination of an acyclic amino acid – Enzyme mechanistic studies aided by a novel microreactor filled with magnetic nanoparticles, CHEMBIOCHEM 16 : 16 pp. 2283-2288. , 6 p. (2015)
33. Boros, Z ; Abaháziová, E ; Weiser, D ; Kovács, P ; Varga, A ; Zaharia, V ; Poppe, L ✉, Surface modification of silica gels for selective adsorption of bacterial lipases, STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 59 : 3 pp. 33-38. , 6 p. (2014)
34. Weiser, D ; Varga, A ; Kovács, K ; Nagy, F ; Szilágyi, A ; Vértessy, B G ; Paizs, C ; Poppe, L ✉, Bisepoxide cross-linked enzyme aggregates – New immobilized biocatalysts for selective biotransformations, CHEMCATCHEM 6 : 5 pp. 1463-1469. , 7 p. (2014)
35. Boros, Z ; Falus, P ; Márkus, M ; Weiser, D ; Oláh, M ; Hornyánszky, G ; Nagy, J ; Poppe, L ✉, How the mode of Candida antarctica lipase B immobilization effects the continuous-flow kinetic resolution of racemic amines at various temperatures, JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 85-86 pp. 119-125. , 7 p. (2013)
36. Boros, Z ; Weiser, D ; Márkus, M ; Abaháziová, E ; Magyar, Á ; Tomin, A ; Koczka, B ; Kovács, P ; Poppe, L ✉, Hydrophobic adsorption and covalent immobilization of Candida antarctica lipase B on mixed-function-grafted silica gel supports for continuous-flow biotransformations, PROCESS BIOCHEMISTRY 48 : 7 pp. 1039-1047. , 9 p. (2013)
37. Ősze, M ; Weiser, D ; Hornyánszky, G ; Poppe, L ✉, Multi-substrate kinetic resolution screening method for lipase biocatalysts, STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 57 : 2 pp. 129-137. , 9 p. (2012)
38. Weiser, D ; Boros, Z ; Hornyánszky, G ; Tóth, A ; Poppe, L ✉, Disubstituted dialkoxysilane precursors in binary and ternary sol-gel systems for lipase immobilization, PROCESS BIOCHEMISTRY 47 pp. 428-434. , 7 p. (2012)
39. Tomin, A ; Weiser, D ; Hellner, G ; Bata, Z ; Corici, L ; Péter, F ; Koczka, B ; Poppe, L ✉, Fine tuning the second generation sol-gel lipase immobilization with ternary alkoxysilane precursor systems, PROCESS BIOCHEMISTRY 46 pp. 52-58. , 7 p. (2011)
40. Weiser, D ; Tomin, A ; Poppe, L ✉, Novel solid supports for lipases in sol-gel immobilization systems, STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 55 : 4 pp. 283-288. , 6 p. (2010)
41. Tomin, A ; Weiser, D ; Bata, Z ; Corici, L ; Péter, F ; Poppe, L ✉, Entrapment of lipases in novel sol-gel systems, STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA 54 : Sp. 2 pp. 77-82. , 6 p. (2009) 2022-03-25 18:37

The NanoBioTechnology group is a subgroup of
BioOrganic Chemistry Research Group  at Dept. of Org.Chem. and Tecnh, and works with strong collaboration with
Soft Matters Research Group at Dept. of Phys.Chem. and Mat.Sci.

Balázs Decsi
Ph.D. student
E-mail: balazsdecsi@edu.bme.hu
Phone: +3614631129

Research
biomimetic reactions and continuous-flow catalysis, analytical knowledge (HPLC, HPLC MS, spectrophotometry)

Gábor Koplányi
Ph.D. student
E-mail: koplanyig@edu.bme.hu
Phone: +3614631129

Research
enzyme immobilization, nanocarrier synthesis and applications, analytical knowledge (SEM, SEM-EDAX, UV-Vis)

Gergő Dániel Tóth
Ph.D. student
Email: tothgergodaniel@edu.bme.hu, Phone: +3614631129

Research
nanofibers, biotransformations, enzyme-based formulations, analytical knowledge (SEM, SEM-EDAX, UV-Vis, rheology)

Réka Farkas
M.Sc. student
E-mail: rekuci.farkas@gmail.com

Research
recombinant enzyme production and purification, enzyme kinetics
and structural characterization

Balázs Kenéz
M.Sc. student
E-mail: kenez.balazs@edu.bme.hu

Research
nanofiber fabrication, biomimetic reactions, production and functionalization of magnetic materials

Levente Kőnig
M.Sc. student
E-mail: konig.levente@edu.bme.hu

Research
nanofiber fabrication, enzyme immobilization,
analysis of biocatalytic reactions

Maximilán Kiss
M.Sc. student
E-mail: kiss.maximilian@edu.bme.hu

Research
enzyme immobilization in nanofibrous material,
characterization of immobilized enzymes

Félix  Nagy
M.Sc. student
E-mail: nagy.felixt@edu.bme.hu

Research
nanocarrier synthesis and
functionalization,
enzyme immobilization, biocatalysis

Alexandra Molnár
B.Sc. student
E-mail molnar.alexandra@edu.bme.hu

Research
nanofiber fabrication
and characterization,
enzyme-based formulations

Dalma Lipták
B.Sc. student
E-mail: liptakdalma@edu.bme.hu

Research
production and functionalization of nanoparticles,
enzyme purification and immobilization

Visegrad Grant – Central European network for wider application of electrospun nanofibers

Nanofibers are an emerging field with applications in filtration and pharmaceuticals (especially in wound dressing, smart implants and drug delivery). The project aims to create a network of excellence in the field of nanofibers in the V4 countries involving three respected academic institutions and industrial partners. The planned activities include a conference and a workshop with industrial experts involved and researcher mobility between the three countries (Poland, Czech Republic and Hungary). 

This project is supported by Visegrad Fund (project ID 223110110)

Development of Lab-on-a-chip platforms for investigation of protein-ligand interaction

Several researches related to the medical and pharmaceutical sciences, aim the synthesis of an increasing number and compounds with high diversity, targeting the deeper understanding of the physiological function of proteins and enzymes and / or to influence and regulate them. Proteins, but in many cases potential protein ligands, can only be produced in limited quantities, so the development of methods and tools suitable for rapid analysis applying small sample sizes can be a key issue. This research is focusing on the development a Lab-on-a-chip-based (LOC) device for ligand binding assays of two selected proteins important for drug discovery, human tyrosine hydroxylase (TH) and human serum albumin (HSA). Continuous flow LOC reactors can eliminate the disadvantages of conventional laboratory methods such as large number of manual operations, higher reagent requirements, or isolation of interfering components. In the case of TH enzyme, potential inhibitor and substrate molecules will be investigated, while in the case of HSA protein both the binding balance of drug compounds and bioactive excipients and the enzymatic function of HSA will be characterized. Integrating LOC-based protein-ligand interactions into a LOC-based reactor can provide new insights for the exploring of functional properties of proteins.

This research is supported by the National Research, Development and Innovation Office (Grant number: FK-137582)

Nanostructured catalyst systems for sustainable biotransformations

The enzymes in their natural or recombinant form have high substrate specificity and high selectivity, thus by enzyme catalysts several optically active compounds can be synthesized efficiently. However, their catalytically active and enantioselective state may be sensitive under the reaction conditions. In addition, biocatalysts are hard to isolate from the homogenous reaction media. Due these disadvantages, the application of native enzymes as catalyst results in serious technological challenges. As the range of enzyme-catalyzed processes extends, more and more complicated reaction conditions and reactor systems may be required. The solid, nanostructured enzyme carriers have unique advantage, that they have large surface area and high stability, and their physico-chemical properties are fine-tunable and adaptable to the requirements of enzyme catalysis. Due to the growing importance of recombinant enzymes and proteins the downstream processes of these valuable biotechnological products cause several technological difficulties. The result of these project can be a novel carrier family and methodology, which are able to isolation, stabilization and immobilization the desired enzyme in parallel way, providing directly applicable biocatalysts.

This research is supported by the National Research, Development and Innovation Office (Grant number: PD-131467)

Assistant Professor at Budapest University of Technology and Economics

MTMT ID
ORCID ID
ResearchGate

Address

Department of Organic Chemistry and Technology, BME)
H-1111 Budapest, Szent Gellért tér 4., Hungary

Department of Physical Chemistry and Materials Scince, BME,
H-1111 Budapest,  Budafoki út 6-8., Hungary

 (36-1)-463-1129

balogh.weiser.diana@vbk.bme.hu­ 

Education, titles

2016: Ph.D. degree (PhD thesis, BME)

2015: Biomedical engineer, M.Sc. (BME – Semmelweis University)

2011: Chemical engineer, M.Sc. (BME)

Professional experiences

2018-: assistant Professor at BME, Department of Organic Chemistry and Technology and Department of Physical Chemistry and Materials Science: Coordination of research projects related to field of biotechnology, pharmaceutical science and materials science, education including lectures and laboratory practices

2015-2018: project manager at Fermentia LLC., coordination of research project related to industrial biotechnology

Research interests

Biotechnology: enzyme immobilization methods, comprehensive analysis of enzymatic reactions and structures and ligand binding, investigation of homogenous and heterogenous biocatalysis in batch and continuous-flow reactors (espically different biotransformation integrated in Lab-on-a-Chip systems), biomimetic systems (modelling of enzymatic functions)

Pharmaceutical sciences: enzyme and protein-based therapies (enzyme replacement therapies, enzyme based solid drug formulations), investigation of enzyme inhibitions and ligand binding by classic and novel Lab-on-a-Chip based methods

Materials sciences: catalyst carrier design including micro- and nanoparticles, porous materials nanofibers, nanotubes and polymer beads, advanced surface modification methods for specific catalyst immobilization or isolation techniques

Teaching activites

Chemistry of Biomolecules (lecture)

Physical chemistry laboratory practice (calorimetry, liquid-vapor phase systems)

Organic chemistry laboratory practice (preparative chemistry)

Non-conventional materials laboratory practice (gels)

Supervisor activities

Principal investigator in research project

2021-2025: Development of Lab-on-a-chip platforms for investigation of protein-ligand interaction (NRDI, FK-137582)

2020-2022: Self-assembling Multi Layer Enzyme Network for Flow Biocatalysis (CELSA-20-243)

2019-2022: Nanostructured catalyst systems for sustainable biotransformations (NRDI, PD-131467)

2019-2022: Development of nanocarrier systems to optimize automated and scalable catalytic syntheses (sub project, NRDI, TKP-NKA-BME)

Supervising 4 PhD student (3 ongoing, 1 absolved) and more than 40 studenst at BSc and MSc level

Awards

János Bolyai Research Grant (by Hungarian Academy of Science: 2021-2024)

New National Excellence Award for Young researcher Bolyai+ (by NRDI, 2021-2022)

Women in Science Excellence Award (by Nature Award-winning Association of Hungarian Women in Science, 2020)

New National Excellence Award for Postdoctoral researcher (by NRDI, 2016-2017)

Scientific publication

3 book chapters, 48 Scientific articles

Total IF: 187.210 — Number of Independent citations: 481
h-index: 16

List of publication: MTMT link

Selected publication

F. Nagy, E. Sánta-Bell, M. Jipa, G. Hornyánszky, A. Szilágyi, K. László, G. Katona, C. Paizs, L. Poppe*, D. Balogh-Weiser*, Cross-Linked Enzyme-Adhered Nanoparticles (CLEANs) for Continuous-Flow Bioproduction, ChemSusChem, 2022, 15, e202102284.

G. Koplányi, E. Sánta-Bell, Z. Molnár, G.D. Tóth, M. Józó, A. Szilágyi, F. Ender, B. Pukánszky, B.G. Vértessy, L. Poppe, D. Balogh-Wesier*, Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning, Catalysts, 2021, 11(10), 1149.

D. Balogh-Weiser*, B. Decsi, R. Krammer, G. Dargó, F.Ender, J. Mizsei, R. Berkecz, B. Gyarmati, A. Szilágyi, R. Tőtős, C. Paizs, L. Poppe, G.T. Balogh, Magnetic Nanoparticles with Dual Surface Functions—Efficient Carriers for Metalloporphyrin-Catalyzed Drug Metabolite Synthesis in Batch and Continuous-Flow Reactors, Nanomaterials, 2020, 10(12), 2329.

D. Weiser, F. Nagy, G. Bánóczi, M. Oláh, A. Farkas, A. Szilágyi, K. László, A. Gellért, G. Marosi, S. Kemény, L. Poppe*, Immobilization engineering – How to design advanced sol-gel systems for biocatalysis? Green Chemistry, 2017, 19(16) 3927-3937.